Tom Ellis Lab
Living Materials from Synthetic SCOBYs
Our lab's paper on Engineered Living Materials (ELMs) with the Tim Lu lab (@MIT) is now published in Nature Materials. Charlie Gilbert did a lot of this work back during his PhD in our group in collaboration with Tzu-Chieh Tang (Zijay) in Tim Lu's group in Boston. Wolfgang Ott and Will Shaw from our group also got involved over the years in this major project that was kickstarted by an MIT-Imperial MISTI Award.
ELMs have previously been created using non-food microbes like E. coli and filamentous fungi, but scalability – the potential for the technology to be produced on a larger scale – has always been a challenge, meaning ELMs aren’t yet widely used. Current ELM technologies also require trained personnel and stringent conditions to grow microbes, hindering their accessibility to the general public. We invented a new type of ELM to solve these problems by taking inspiration from the natural symbiotic approach of the kombucha SCOBY and combining genetically engineered yeast cells with cellulose-producing bacteria, making a ‘Syn-SCOBY’.
The Syn-SCOBY-produced cellulose acts as a scaffold which can hold the multi-functional enzymes produced by the yeast. This combination led us to be able to make programmable and tough materials easily at a large scale from cheap sugar mixtures. Most importantly, because almost any version of yeast modified in the lab can be immediately used to produce kombucha-inspired materials in our system, dozens of different cell engineering options can be achieved in a ‘plug and play’ manner. We created a material incorporating yeast that senses estradiol, a hormone which is sometimes found as an environmental pollutant, and produces GFP signal in response. We also engineered materials where the yeast within them secrete enzymes that break down antibiotics, pollutants and even the cellulose itself. Finally we even engineered the living material to respond to light and glow with luciferase in response.
The research was funded in part by the UK Engineering and Physical Sciences Research Council (EPSRC), U.S. Army Research Office, the MIT Institute for Soldier Nanotechnologies, and the MIT-MISTI MIT-Imperial College London Seed Fund.
Charlie Gilbert, Tzu-Chieh Tang, Wolfgang Ott, Brandon A. Dorr, William M. Shaw, George L. Sun, Timothy K. Lu, Tom Ellis (2021): Living materials with programmable functionalities grown from engineered microbial co-cultures. Nature Materials. https://doi.org/10.1038/s41563-020-00857-5